Best Practices for High-content Screening with Cell Painting:
- Image Acquisition:
- Use water immersion objectives and high NA lenses for sharp images.
- Employ large sCMOS detectors for high sensitivity and dynamic range.
- Utilize confocal imaging with Maximum Intensity Projection for clearer details.
- Optimize signal-to-background ratio by adjusting laser power and exposure.
- Data Acquisition:
- Standardize image and data acquisition protocols for consistency.
- Use Yokogawa CellVoyager CV8000 for optimal confocal acquisition.
- Minimize background noise and photobleaching for accurate analysis.
- Analysis:
- Generate high-quality cellular feature data for precise analysis.
- Enhance drug discovery and disease modeling with reliable phenoprints.
