About this webinar
Genome modification has now become an essential part of most Biotechs research and development pipelines. This is done to produce new cell lines that convey a modification such as a fluorescently labelled target protein, knock out of a target or mutation of a target.
Two general approaches are commonly used: electroporation of nuclease and nucleic acids i.e. Cas9 RNPs or transfection of vectors expressing nucleases, targeting nucleic acids and donor genetic material. One of the major limitations of these approaches is that they take a long time to generate a usable clonal cell population due to the need for selection, population expansion usually with some type of cell sorting, a process that can often take several months time. In addition, electroporation or transfection simply does not work for some cell types especially when attempting to insert large DNA fragments such as genes encoding full length fluorescent proteins, where correct insertion rates can drop below 0.01%.
Nanopipette delivery offers an alternative method of delivering genome editing material such as Cas9 RNPs with donor DNA. A major advantage of Yokogawa's SU10 nanopipette delivery system is the ability to efficiently genome edit cells at a single cell level, allowing the user to start with a clonal population and decreases cell line generation time to as fast as ~3 weeks. In this webinar I will talk about my experience with the SU10 system, describing my impressions and how, in my opinion, it can be best utilized in a small biotech setting.
Learning outcomes of this webinar:
Speaker: Dr. Andrew Seeber
Director Cellular Assays
Transition Bio
Watch the webinar now!